Human activities and their interaction with natural environment shape the dissemination of antibiotic resistance. Research on antibiotic resistance (AR) in Indian context has been done in hospital settings focusing mainly on clinical pathogens, neglecting the enormous contribution from natural environments. Our metagenomic scale work on sewage sample would provide information from both human gut and external environment, thus giving a complete status of microbial community structure. When coupled with functional metagenomics, we would specifically identify genes that provide resistance against broad spectrum antibiotics. Our focus is to generate qualitative and quantitative data on antibiotic resistant microbes, which would give an estimate of resistant pathogenic load and dissemination of antibiotic resistance in non-pathogenic and otherwise beneficial microbes. The status of resistance marker genes would be used to associate with the prevalence of antibiotic resistant gut microflora. Our results would identify focal points of antibiotic resistance transfer at the intersection of humans and environment. Thus, our findings would prove as vital aid in design of risk assessment strategy and containment of multi-drug resistant pathogens which have raised grave concerns lately.