Dysbiosis of intestinal microflora has been observed in Ulcerative colitis (UC), which is characterized by a shift of bacterial communities due to the alteration in their environment. Although this is true, the changes in mucosal microflora during different stages of UC are largely unknown. Our study aims to investigate the changes in mucosal tissue associated microbiota during various phases of Ulcerative Colitis (UC). The study involves understanding the mucosal microbiota associated with colon biopsy and also fecal samples of patients suffering from UC (exacerbated stage) and the follow-up samples from the same patients (remission stage), as well as non-IBD subjects (Walujkar et al., 2014). Our study helps in identifying the specific genera dominating the microbiota during the disease and thus, lays a basis for further investigation of the role of these bacteria in the pathogenesis of UC.