Dubbed as “pristine” habitats as far as Microbiome goes, tribal populations can throw some wonderful insights into the harmony of hosts and microbes. Interesting questions like human evolutionary lineage and even some unique microbes that are perhaps lost from the urban population can be probed while studying microbiomes of these populations. With an aim to delve into insights for pristine microbiomes, the tribal population of Rongmei, located in Manipur was selected for this study. Such endogamous groups, who are far away from the urban lifestyle have been residing for centuries in the same place, have dietary intake based on the available food resources and have cultural practices that are a defining feature for their identity. With our limited knowledge, we can predict that these factors may have led for a unique microbiome which endows the host with all essential health features.