Studies on the Indian microbiome have indicated a notable difference from populations over the world due to its diverse genetic composition, dietary habits, cultural, religious affiliations and vast geographic scattering. Population groups with particular microbiome demands specific nutritional needs and thus constitute an opportunity for the development of probiotics that fulfil the requirements of such groups. Additionally, a probiotics import in Indian market is observed to be much higher, however their population-wide understanding is deficient in terms of efficacy and ability to deliver health benefits. In order to achieve the aim of population-specific probiotic bacterium, we isolated bacterial strains from faecal samples of healthy Indian subjects and screened for their probiotic potentials. More than 125 different strains belonging to 18 different genera were isolated using 35 culture media combinations. We employed an integrated approach of in-vitro experiments, genomic and comparative genomics analysis to characterize the metabolic characters of strain 17OM39, to claim its inherent potential of being probiotic.